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. 2010 Apr 12;5(4):e10127. doi: 10.1371/journal.pone.0010127

Figure 3. T cell and B cell numbers show an age-dependent decline in the lymphoid periphery of Lmna-/- mice.

Figure 3

Splenocytes were surface stained for CD4, CD8, CD19, CD62L, CD44, and panTCRβ. A. Representative flow cytometry plots show the percent of CD4+ and CD8+ T cells. B. Absolute numbers of splenic CD4+ and CD8+ T cells for each indicated age group. C. Gated CD4+ and CD8+ splenocytes were analyzed for CD62L and CD44 expression. D. Representative histograms showing the percent of CD19+ B cells in the spleen. E. Absolute numbers of CD19+ B cells per spleen compiled for each indicated age group. Numbers in plots and histograms indicate the percentage of each gated population. Charts show the mean cell number with error bars indicating the standard deviation (Lmna+/+ N = 3; Lmna-/- N = 3 for each indicated age group). P values were calculated using an unpaired two-tailed Student's t test (* p<0.05, ** p<0.001).