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. 2010 Apr 12;5(4):e10127. doi: 10.1371/journal.pone.0010127

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Hale et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bone marrow single-cell suspensions were stained for surface B220, IgM, and IgD expression. A. Absolute number of bone marrow cells. B. Representative histograms from 9-week-old mice showing the percent of bone marrow cells that are developing B220⁺ cells. C. The percent of bone marrow cells that are B220⁺ for each indicated age group. D. Absolute number of B220⁺ bone marrow cells for each indicated age group. E. Representative IgM/IgD plots of gated B220⁺ bone marrow cells from 9-week-old mice. F. Absolute number of B220⁺ bone marrow cells that are IgM^-IgD^-, IgM⁺IgD^- (immature B cells), and IgM⁺IgD⁺ (mature B cells). Numbers in plots and histograms indicate the percentage of each gated population. Charts show the mean value with error bars indicating the standard deviation (Lmna^+/+ N = 3; Lmna^-/- N = 3 for each indicated age group). P values were calculated using an unpaired two-tailed Student's t test (* p<0.05, ** p<0.001).