Fig. 1.

Mice were immunized s.c. on days 0, 7, 14 and 21 with OVA (arrows pointing up). Intranasal OVA challenges were administered on days 27, 29 and 31, and then repeated twice a week for 1 month. Different groups of mice were either administered ETS alone or ETS in combination with OVA challenges. ETS was started on day 33 (after mice had been sensitized and challenged on 3 occasions with intranasal OVA). The ETS was continued 5 days/week for 1 month. ISS was administered on 3 occasions i.p. (arrows pointing down) starting on day 26 with repeat doses on days 32 and 46. The mice were sacrificed 24 h after the final OVA challenge on day 60 and BAL fluid and lungs were analyzed.