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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Anal Biochem. 2009 Dec 14;398(2):203–211. doi: 10.1016/j.ab.2009.12.020

Figure 5. Confirmatory Dose Response Assay.

Figure 5

Cells grown to mid-log phase were stained with 50 μM BCECF-AM and 5 μl aliquots distributed into 384-well plates containing varied amounts of the hit compound disulfiram. Plates (0.25 OD600 cells /μl) were incubated for 90 min at 30 °C. After excitation at 488 nm, fluorescence was collected using a 530/40 nm filter set and a CYAN ADP (Beckman-Coulter) flow cytometer. Data files were processed using IDLQuery/HyperView software programs. The Y axis displays the amount of fluorescence (FL1 Median Channel Fluorescence). WT=wild-type, vma2Δ=V-ATPase null mutant cells.