Table 1.
analyte | efficiency (× 105 theoretical plates) | peak height (av ± SD)b | peak area (av ± SD)c | elution time(s) (av ± SD)d |
---|---|---|---|---|
SphFl | 1.9 | 0.80 ± 0.02 | 2.94 ± 0.05 | 320 ± 14 |
SphFl-1-P | 1.8 | 0.40 ± 0.03 | 1.04 ± 0.07 | 246 ± 8 |
The average ± standard deviations (n = 9) are given for the normalized peak heights, peak areas, and elution times using Bodipy-Fl as the internal standard.
The peak height was that of the second isomer peak.
The peak area was calculated for both of the isomer peaks.
The migration time was the average migration time of the two isomer peaks.