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Effect of HIV-1 clade B and C Tat protein on IDO protein expression. Astrocytes (1 × 106 cells/ml) were separately treated with heat-inactivated (HI) control and HIV-1 clade B and C Tat at 50 ng/ml for 48 h and analyzed by Western blot. (A) A representative experiment: lane 1, control; lane 2, Tat B; lane 3, Tat C. Data presented in (B) are the mean ± SD of three independent experiments.
