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Mapping of the MAb 98-6 epitope. (A) The sgp140(-) glycoproteins from the JR-FL, YU2, and HXBc2 strains of HIV-1 were precipitated by PS or MAb NC-1 98-6. (B) The HXBc2 sgp140(-) mutants truncated immediately C-terminal to the indicated amino acid residues were precipitated by MAb 98-6. (C) The wild-type (WT) HXBc2 sgp140(-) glycoproteins or mutants containing the indicated changes were precipitated by PS or MAb 98-6. The precipitates were analyzed by SDS-PAGE.
