FIG. 2.

SIVsmmFGb stock virus sequence group composition. As described in Fig. 1 and Materials and Methods, 16 SIVsmmFGb stock virus templates, with different RNA and DNA dilutions, were used for nested PCR reactions of the env V1 region, nef, and int. At least 20 clones were sequenced and translated for each gene, yielding approximately 400 total SIVsmmFGb amino acid sequences per gene. These sequences were grouped as described in Fig. 1 and the number and percentage of sequences in each group are shown for (A) the Env V1 region, (B) Nef, and (C) Int.