Table 1.

Description and Sequences of Primers Used for PCR Amplification of env, nef, and int Genes from Tissue Proviral DNA

Primer number	Primer coordinatesa	Primer description	Primer sequence (5′ to 3′)
952	6360	env outer forward	GAGGCGTGCTCTAATACAT
953	9299	env outer reverse	ATCATCATCATCTACATCATC
954	6474	env inner forward	CCGAAGAAGGCTAAGGCTAATACA
955	9210	env inner reverse	ATTGTCCCTCACTGTATCCCTG
959	8806	nef outer forward	AGACGGTGGAGACAGAGGTGG
960	9950	nef outer reverse	TCAATCTGCCAGCCTCTCCG
961	8947	nef inner forward	CCAACCAGTGTTCCAGAGGC
962	9845	nef inner reverse	CCCGTAACATCCCCTTTGTGG
963	4021	int outer forward	CCTCCCTTAGTCAGATTAGTC
964	5433	int outer reverse	GGGAAGATTACTCTGCTG
965	4106	int inner forward	GGCAATCAAGAGAAGGAAAGGC
966	5378	int inner reverse	CATAACAAGCCTTCTGTAGGTCC

^aPrimer coordinates based on PGm5.3 genome.⁴