Fig. 1.
ΔNp63 expression analysis during hair follicle development and cycling. (A,B) At E14.5, expression of ΔNp63 protein can be detected in the developing placodes and continues to be expressed in the down-growing hair germ at E16.5 (B). Dotted lines indicate the epidermal-dermal junction. (C) By postnatal day 1 (P1), ΔNp63 is expressed along the entire length of the outer root sheath (ORS) as well as the matrix cells. (D) At P21 (telogen), ΔNp63 expression is localized to the basal layer, ORS and the bulge region (brackets, insets) of the hair follicle and secondary hair germ (Hg). (E,F) Co-staining with K15 and Sox9 reveals co-localization of ΔNp63 to hair follicle stem cells. Scale bars: 50μm.