Fig. 1.
BPA and E2 protect T47D cells from cisplatin cytotoxicity. (A) Cells were treated with 1 nM BPA or E2 for 24 hrs, followed by incubation with increasing concentrations of cisplatin (0–800 ng/ml) for 72 hrs. (B) T47D cells were treated with increasing concentrations of BPA (0–10nM) for 24 hrs prior treatment with 800 ng/ml cisplatin for 24 hrs. (C) T47D cells were treated with increasing concentrations of E2 (0–10nM) for 24 hrs before treatment with 800 ng/ml cisplatin for 24 hrs. In all cases, cell viability was determined by the MTT assay. Each value is a mean±SEM of six replicates. In (A) * significant (p< 0.05) vs cisplatin alone. In (B&C) * significant vs control, and ** significant vs cisplatin.