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. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: Hepatology. 2009 Apr;49(4):1277–1286. doi: 10.1002/hep.22743

Fig. 8.

Fig. 8

Forced increase in p-Erk provides a survival advantage in CD133− cells. Adenovirus vectors with β-Gal or CA-MEK1 were used at different MOI. (A) Western blot of p-Erk and pan-Erk demonstrated a marked increase in p-Erk levels in cells infected with CA-MEK1 adenovirus compared with β-Gal adenovirus, with increased levels at higher MOI. (B) Graph of densitometry analysis of p-Erk/pan-Erk ratio demonstrates a marked increase in the p-Erk/pan-Erk ratio with increasing MOI of CA-MEK1 adenovirus. (C) CA-MEK1 confers a survival advantage in CD133− cells after TGF-β stimulation (2 × 104 cells/cm2 plated annexin V/PI FACS analysis, n = 3, 50,000 events/replicate + standard error). #1,#2,#3P < 0.05.