Figure 5.

Immunofluorescence staining of P5 control and Dicer CKO retinas. A–H, Retinal paraffin sections. YFP staining (green) indicates areas of cre-mediated recombination and Dicer CKO. A, B, Retinal progenitor Sox9 staining (red) is reduced in Dicer-deficient areas (arrowheads), whereas Tuj1⁺ neurons (blue) are ectopically located. C, D, Otx2⁺ photoreceptors (red) are reduced in Dicer-deficient areas (arrowheads), whereas bright Pax6⁺ amacrine cells are absent (blue). Faint Pax6 staining in RPCs is increased in Dicer-deficient regions. E, F, Mature rhodopsin⁺ rods (red) are absent from Dicer-deficient areas (arrowheads). G, H, Müller glia, stained with Cralbp (red) and Glast (blue), are absent from Dicer-deficient areas (arrowheads). NBL, Neuroblastic layer; INL, Inner nuclear layer; GCL, ganglion cell layer. All images are oriented the same, with the ganglion cell layer on the bottom and peripheral retina to the right. Boxes represent areas of magnification. Scale bars, 100 μm.