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. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: Dev Biol. 2008 Dec 3;327(1):12–23. doi: 10.1016/j.ydbio.2008.11.015

Fig. 3. RNAi of plp-1 reduces end-1 expression.

Fig. 3

(A–D) PLP-1 staining is diminished or eliminated by RNAi of plp-1 (B and D; compare with wild type, panels A and C). Antibody to PGL-1 (Kawasaki, et al., 1998), a component of the germline-specific P-granules, was used as a positive control for permeabilization (arrowheads).

(E–H) Anti-β-galactosidase antibody staining of the same embryos expressing an end-1 reporter. plp-1(RNAi) embryos show reduced expression of the reporter at the 2E stage (F) and 4E stage (H) compared to wild type (E and G).

(I–L) The same embryos as in (A–H) stained with DAPI to reveal nuclei.

(M) Distribution of embryos expressing end-1 at levels that appear normal (wt), strongly reduced, and undetectable (off). plp-1(RNAi) embryos that that were positive for P granule staining and that showed loss of PLP-1 immunostaining (to compensate for impenetrant reduction of plp-1) were scored for end-1 expression. The results were summed over eight experiments in total (143 wild-type control embryos and 138 plp-1(RNAi) embryos). Error bars indicate standard error of the mean.