Fig. 6. PLP-1 nuclear localization requires lit-1 and mom-4 function.
(A–D) PLP-1 staining in Wnt and MAPK mutants. PLP-1 is at higher levels in the nucleus than in the cytoplasm in wildtype (Fig. 3), mom-2, and mom-4 mutant embryos (A and B). Cytoplasmic levels of PLP-1 are increased in lit-1(RNAi) embryos (C). PLP-1 is largely excluded from the nucleus in lit-1(RNAi); mom-4 double mutants (D). (E–H) Staining with MPM2 control, showing very little or no effect on nuclear localization of the nuclear protein recognized by this antibody.