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. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: J Org Chem. 2009 Feb 6;74(3):1070–1081. doi: 10.1021/jo802037v

Table 1.

Thermal denaturation data for N2′-Functionalized 2′-amino-α-L-LNA and reference strands against DNA complements.a

Tm [ΔTm/mod] (°C)
ON Duplex B = T O Q S V W X Y Z
B1 5′-GBG ATA TGC 28.5 31.0 26.5 20.0 17.5 35.5 38.5 39.0 29.0
D2
3′-CAC TAT ACG
  
  
 [+2.5]
 [–2.0]
 [–8.5]
 [–11.0]
 [+7.0]
 [+10.0]
 [+10.5]
 [+0.5]
B2 5′-GTG ABA TGC 28.5 34.5 29.0 20.0 16.5 42.5 47.5 44.0 34.5
D2
3′-CAC TAT ACG
  
  
 [+6.0]
 [+0.5]
 [–8.5]
 [–12.0]
 [+14.0]
 [+19.0]
 [+15.5]
 [+6.0]
B3 5′-GTG ATA BGC 28.5 31.5 27.5 16.5 14.5 39.0 42.5 40.0 ND
D2
3′-CAC TAT ACG
  
  
 [+3.0]
 [–1.0]
 [–12.0]
 [–14.0]
 [+10.5]
 [+14.0]
 [+11.5]
  
D1 5′-GTG ATA TGC 28.5 32.0 28.0 17.0 12.0 35.0 39.0 38.5 29.0
B4
3′-CAC BAT ACG
  
  
 [+3.5]
 [–0.5]
 [–11.5]
 [–16.5]
 [+6.5]
 [+10.5]
 [+10.0]
 [+0.5]
D1 5′-GTG ATA TGC 28.5 36.5 31.0 22.5 19.0 44.0 48.0 45.0 35.0
B5
3′-CAC TAB ACG
  
  
 [+8.0]
 [+2.5]
 [–6.0]
 [–9.5]
 [+15.5]
 [+19.5]
 [+16.5]
 [+6.5]
D1 5′-GTG ATA TGC 28.5 36.0 27.5 <10 <10 ND 53.5 55.5 ND
B6
3′-CAC BAB ACG
  
  
 [+3.8]
 [–0.5]
  
  
  
 [+12.5]
 [+13.5]
  
B7 5′-GBG ABA BGC 28.5 36.0 27.0 <10 <10 ND ND 69.0 ND
D2 3′-CAC TAT ACG [+2.5] [–0.5] [+13.5]
a

Thermal denaturation temperatures [Tm values/°C (ΔTm = change in Tm value calculated relative to D1:D2, D1:R2 and R1:D2 reference duplexes)] measured as the maximum of the first derivative of the melting curve (A260 vs temperature) recorded in medium salt buffer ([Na+] = 110 mM, [Cl–] = 100 mM, pH 7.0 (NaH2PO4/Na2HPO4)), using 1.0 μM concentrations of the two complementary strands. Tm values are averages of at least two measurements; A = adenin-9-yl DNA monomer, C = cytosin-1-yl DNA monomer, G = guanin-9-yl DNA monomer, T = thymin-1-yl DNA monomer, O = α-L-LNA thymin-1-yl monomer (Fig. 1). For structures of monomers Q-Z see Figure 1. ND = not determined.