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. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: J Org Chem. 2009 Feb 6;74(3):1070–1081. doi: 10.1021/jo802037v

Table 2.

Thermal denaturation data for N2′-functionalized 2′-amino-α-L-LNA and reference strands against RNA complements.a

Tm [ΔTm/mod] (°C)
ON Duplex B = T O Q S V W X Y Z
B1 5′-GBG ATA TGC 26.5 32.5 27.5 19.5 19.0 27.0 32.5 32.5 26.5
R2
3′-CAC UAU ACG
  
  
 [+6.0]
 [+1.0]
 [–7.0]
 [–7.5]
 [+0.5]
 [+6.0]
 [+6.0]
 [±0]
B2 5′-GTG ABA TGC 26.5 35.0 28.5 18.5 17.0 31.5 36.5 36.0 33.5
R2
3′-CAC UAU ACG
  
  
 [+8.5]
 [+2.0]
 [–8.0]
 [–9.5]
 [+5.0]
 [+10.0]
 [+9.5]
 [+7.0]
B3 5′-GTG ATA BGC 26.5 32.0 28.0 19.0 16.5 28.0 33.5 32.5 ND
R2
3′-CAC UAU ACG
  
  
 [+5.5]
 [+1.5]
 [–7.5]
 [–10.0]
 [+1.5]
 [+7.0]
 [+6.0]
  
R1 5′-GUG AUA UGC 24.5 31.0 27.5 19.5 17.5 23.5 29.0 30.5 19.5
B4
3′-CAC BAT ACG
  
  
 [+6.5]
 [+3.0]
 [–5.0]
 [–7.0]
 [–1.0]
 [+4.5]
 [+6.0]
 [–5.0]
R1 5′-GUG AUA UGC 24.5 34.5 29.0 21.5 20.5 32.0 36.0 36.5 31.0
B5
3′-CAC TAB ACG
  
  
 [+10.0]
 [+4.5]
 [–3.0]
 [–4.0]
 [+7.5]
 [+11.5]
 [+12.0]
 [+6.5]
R1 5′-GUG AUA UGC 24.5 35.5 28.5 <10 <10 ND 39.0 44.0 ND
B6
3′-CAC BAB ACG
  
  
 [+5.5]
 [+2.0]
  
  
  
 [+7.3]
 [+9.8]
  
B7 5′-GBG ABA BGC 26.5 44.0 31.0 <10 <10 ND ND 52.5 ND
D2 3′-CAC UAU ACG [+5.8] [+1.5] [+8.7]
a

For conditions of thermal denaturation experiments see Table 1.