Table 4.
Complementary DNA |
Complementary RNA |
||||||
---|---|---|---|---|---|---|---|
ON | Sequence | ΔG298 [ΔΔG298] (kJ/mol) | ΔH [ΔΔH] (kJ/mol) | T298ΔS [Δ(T298ΔS)] (kJ/mol) | ΔG298 [ΔΔG298] (kJ/mol) | ΔH [ΔΔH] (kJ/mol) | T298ΔS [Δ(T298ΔS)] (kJ/mol) |
D1 | 5′-GTG ATA TGC | –41 | –327 | –286 | –38 | –275 | –237 |
O2 | 5′-GTG AOA TGC | –46 [–5] | –354 [–27] | –308 [–22] | –46 [–8] | –361 [–86] | –315 [–78] |
Q2 | 5′-GTG AQA TGC | –41 [0] | –312 [+15] | –271 [+15] | –40 [–2] | –302 [–27] | –261 [–24] |
S2 | 5′-GTG ASA TGC | –33 [+8] | –349 [–22] | –316 [–30] | –31 [+7] | –352 [–77] | –321 [–84] |
V2 | 5′-GTG AVA TGC | –29 [+12] | –295 [+32] | –266 [+20] | –30 [+8] | –303 [–28] | –273 [–36] |
Thermal denaturation curves were obtained as described in Tables 1 and 2. ΔΔG298, ΔΔH and Δ(T298ΔS) change in ΔG298, ΔH and Δ(T298ΔS) values, respectively, calculated relative to D1:D2 and D1:R2 reference duplexes). Values in italics indicate deviation from (or lack of) monomer trend. ND = not determined. See Table S6 for data from B1-B5 series.