Table 6.
Pyrene absorption maxima for single stranded pyrene-functionalized 2′-amino-α-L-LNA and the corresponding duplexes with DNA/RNA complements.a
|
λmax (Δλmax)/ nm |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| B = |
W |
X |
Y |
Z |
||||||||||
| ON | Sequence | ss | + DNA | + RNA | ss | + DNA | + RNA | ss | + DNA | + RNA | ss | + DNA | + RNA | |
| B1 | 5′-GBG ATA TGC | 348 | 350 [+2] | 349 [+1] | 349 | 351 [+2] | 351 [+2] | 350 | 351 [+1] | 352 [+2] | 346 | 348 [+2] | 348 [+2] | |
| B2 | 5′-GTG ABA TGC | 347 | 351 [+4] | 349 [+2] | 348 | 353 [+5] | 351 [+3] | 346 | 351 [+5] | 351 [+5] | 346 | 347 [+1] | 346 [±0] | |
| B3 | 5′-GTG ATA BGC | 348 | 351 [+3] | 350 [+2] | 350 | 354 [+4] | 351 [+1] | 348 | 351 [+3] | 351 [+3] | ND | ND | ND | |
| B4 | 3′-CAC BAT ACG | 348 | 350 [+2] | 348 [±0] | 348 | 351 [+3] | 349 [+1] | 350 | 351 [+1] | 351 [+1] | 346 | 347 [+1] | 346 [±0] | |
| B5 | 3′-CAC TAB ACG | 348 | 350 [+2] | 349 [+1] | 348 | 351 [+3] | 351 [+3] | 346 | 351 [+5] | 352 [+6] | 346 | 348 [+2] | 347 [+1] | |
a
Measurements were performed at room temperature on a spectrophotometer in the range 300-400 nm, using a quartz optical cell with a 1.0 cm path length and same conditions as for thermal denaturation experiments.