Figure 8.

CaMKII involvement in the DTT-mediated enhancement of NMDAR synaptic responses in aged animals. A–D, Time course of the change in the normalized NMDAR-fEPSP slope in the aged animals that were incubated with the broad spectrum Ser/Thr kinase inhibitor H-7 dihydrochloride (10 μm, n = 7) (A), the CaMK inhibitor KN-62 (10 μm, n = 5) (B), the specific CaMKII inhibitor myr-AIP (5 μm, n = 4) (C), or the PKC inhibitor Bis-I (500 nm, n = 6) (D) before and during DTT application. E, Quantification of the mean percentage change in the NMDAR-fEPSP slope for aged animals under DTT alone (filled bar), and DTT applied in the presence of H-7, KN-62, myr-AIP, and Bis-I (gray bars). An asterisk indicates a significant difference (p < 0.05) between the increases observed in presence of DTT alone and DTT applied in the presence of H-7, KN-62, myr-AIP, and Bis-I. A pound sign indicates a significant (p < 0.05) increase in the response relative to baseline level of 100%, following DTT application.