Fig. 3.

A. Characterization of the goat anti-human Cp antibody against human, mouse, and rat plasma. Plasma, 1 μL, was subjected to denaturing SDS PAGE, 8% gel, and probed for Cp. The blot was stripped and reprobed for transferrin (Tf). A single band, MW 135 kDa, was detected in human, CuA rat and mouse and Cp +/+ mouse. No Cp band was detected in the plasma from Cp -/- mice but a robust band for Tf was present. B. Abundance of Cp and Tf in mouse plasma following copper deficiency. Each lane contained 1 μL plasma from CuA or CuD mice. A 60% reduction in Cp abundance was detected in CuD mouse samples, P < 0.05, whereas Tf levels were comparable.