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. 2010 Mar 5;25(5):1271–1279. doi: 10.1093/humrep/deq040

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© The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which, permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Morphology of hESCs HS293 and HS306 colonies. (A) Typical colonies of cell lines HS293 and HS306 in magnification ×40 and ×400 using phase contrast objective (Ph) showed normal morphology with typical characteristics of human embryonic stem cells (hESCs) with large nuclei with surrounding cytoplasm, the cells being packed tightly together. (B) Colony formation of cell line HS293 and HS306 (×40 and ×400 Ph) after thawing cells which had been frozen with STEM-CELLBANKER and thawed with CELLOTION, 3 days after thawing, showing normal hESC morphology. (C) Colony formation (×40 and ×400 Ph) after thawing hESCs which had been frozen in 10% DMSO in serum replacement (SR)-containing medium. Colonies are growing in clumps, with a very small outgrowth of normal hESCs by the boarders of the cell clump.

Morphology of hESCs HS293 and HS306 colonies. (A) Typical colonies of cell lines HS293 and HS306 in magnification ×40 and ×400 using phase contrast objective (Ph) showed normal morphology with typical characteristics of human embryonic stem cells (hESCs) with large nuclei with surrounding cytoplasm, the cells being packed tightly together. (B) Colony formation of cell line HS293 and HS306 (×40 and ×400 Ph) after thawing cells which had been frozen with STEM-CELLBANKER and thawed with CELLOTION, 3 days after thawing, showing normal hESC morphology. (C) Colony formation (×40 and ×400 Ph) after thawing hESCs which had been frozen in 10% DMSO in serum replacement (SR)-containing medium. Colonies are growing in clumps, with a very small outgrowth of normal hESCs by the boarders of the cell clump.