Figure 5.
The effects of AS160-4P on GLUT4 accumulation in the evanescent field and the fraction of this GLUT4 inserted into the PM. The effects of AS160-4P on GLUT4 translocation to the PM (αHA Cy3EPI/GFPEPI fluorescence ratio; A), the insertion efficiency (fraction of GLUT4 in the evanescent field that is inserted into the PM (αHA Cy3TIRF/GFPTIRF fluorescence ratio; B), and accumulation in evanescent field (GFPTIRF/GFPEPI fluorescence ratio; C) in unstimulated and insulin-stimulated adipocytes. Adipocytes were transiently transfected with WT HA-GLUT4-GFP and AS160-4P the day before the experiment. The data are the means of four experiments ± SEM; #p < 0.05 compared with basal control; *p < 0.05 compared with insulin-stimulated control.