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. 2010 Apr 15;21(8):1375–1386. doi: 10.1091/mbc.E09-08-0751

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© 2010 by The American Society for Cell Biology

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Figure 6. — Characterization of FA/EE-GLUT4 mutant accumulation in the evanescent field and fraction of this mutant inserted into the PM. Characteristics of F⁵A/EE^499,501AA HA-GLUT4-GFP translocation (αHA Cy3^EPI/GFP^EPI fluorescence ratio; A), insertion efficiency (fraction of GLUT4 in the evanescent field that is inserted into the PM; αHA Cy3^TIRF/GFP^TIRF fluorescence ratio; B), and accumulation in evanescent field (GFP^TIRF/GFP^EPI fluorescence ratio; C) in unstimulated and insulin-stimulated adipocytes. Cells were treated with DMSO, 1 μM Akti1/2, or 100 nM wortmannin 30 min before and during insulin stimulation. The data are averages ± SEM from four to five independent experiments normalized to the values for WT GLUT4 in the basal state. *p < 0.05 compared with the control in the basal state; ^#p < 0.05, ^##p < 0.001 compared with the control in the insulin-stimulated state; ^Φp < 0.05.