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. 2010 Apr 15;21(8):1375–1386. doi: 10.1091/mbc.E09-08-0751

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© 2010 by The American Society for Cell Biology

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Figure 9. — Characterization of GLUT4 accumulation in the evanescent field and fraction of this GLUT4 that is inserted into the PM in insulin-resistant adipocytes. Characteristics of WT HA-GLUT4-GFP translocation (αHA Cy3^EPI/GFP^EPI fluorescence ratio; A),“insertion efficiency (fraction of GLUT4 in the evanescent field that is inserted into the PM; αHA Cy3^TIRF/GFP^TIRF fluorescence ratio; B), and accumulation in evanescent field (GFP^TIRF/GFP^EPI fluorescence ratio; C) in unstimulated and insulin-stimulated control and insulin-resistant adipocytes. 100 nM wortmannin was included as noted. The results are averages ± SEM from three to nine experiments, *p < 0.05; **p < 0.001.