Figure 2.

Colocalization of GFP-fused PKCε C1b and β2-chimaerin C1 domains with p23/Tmp21. HeLa cells were cotransfected with pEGFP-fused C1εa, C1εb, C1ζ, or C1β2-ch (or empty vector) and V5-tagged full-length pcDNA3-p23/Tmp21. Forty-eight hours later, cells were fixed and stained with an anti-V5 antibody, and localization was examined by confocal microscopy. Colocalization images and Pearson's r (Rr) were generated by ImageJ. Similar results were observed at least in three independent experiments. Bar, 10 μm.