Figure 5. Ebselen and SID 17387000 inhibit TbHK activity from parasite cell lysate and cause a reduction in cellular G6P levels in BSF parasites.

Increasing amounts of (A.) ebselen or (B.) SID 17387000 were incubated with 2×10⁵ BSF cell equivalents for 15 min at RT and HK assays were performed as described in the Materials and Methods. (C.) Growth in the presence of Ebselen or SID 17387000 causes a reduction in cellular G6P levels. BSF parasites (1×10⁷) were cultured for 1 or 4 hours in the presence of 30 µM or 1 µM (10-fold the EC₅₀) ebselen or SID 17387000 followed by lysate preparation and comparison of G6P levels to an equivalent number of untreated parasites [14].