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. 2010 Apr 13;8(4):e1000355. doi: 10.1371/journal.pbio.1000355

Figure 5. Subchronic and chronic antidepressant effects of Spadin.

Figure 5

Subchronic treatments: Spadin (10−6 M), Fluoxetine (3 mg/kg), or Saline solutions were i.v. injected in a 100 µL bolus once a day for 4 successive d before the test. In chronic treatments, spadin (10−6 M) and fluoxetine (1 mg/kg) were i.v. injected in a 100 µL bolus once a day for 15 successive d. For each test there were 8 animals per group. (A) In FST (one-way ANOVA, F 2,23 = 26.08, ***p<0.001) and (B) TST (one-way ANOVA, F 2,24 = 9.8, *p<0.05 versus saline-treated mice), spadin induced similar behaviors than those obtained with the acute treatment, whereas fluoxetine was without effect [30]. (C) In FST, chronic treatment with spadin or fluoxetine significantly reduced the time of immobility (one-way ANOVA, F2,26 = 25.08, ***p<0.001 versus saline-treated mice). (D) NSF paradigm: at the end of the 4 d treatment, animals were food deprived for 1 d and then measured for their latency to feed. Spadin treatment significantly reduced the latency to feed when compared to saline or fluoxetine treatments (t test, ***p<0.001 versus saline-treated mice). In all graphs, data are expressed as means ± SEM.