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. 2010 Apr 13;8(4):e1000355. doi: 10.1371/journal.pbio.1000355

Figure 6. Effect of Spadin on stress and anxiety behaviors.

Figure 6

(A) Decreased stress-induced serum levels of corticosterone in mice treated with spadin. We compared serum corticosterone concentrations (ng/ml) sampled in the morning in mice acutely treated with spadin (i.v, 10−6 M), saline or fluoxetine (i.p., 3 mg/kg) 30 min after a 10 min tube restraint (n = 10 per group). Data are expressed as increase of the ratio corticosterone levels 30 min after stress over basal corticosterone levels 30 min before restraint (one-way ANOVA, F2,27 = 18.30, *p<0.05, **p<0.01 versus saline-treated mice). (B) Effect of spadin (i.p, 10−5 M) and diazepam (i.p., 0.5 mg/kg) on time spent in the open arms (s) of the elevated plus-maze (n = 10 per group, one-way ANOVA, F2,27 = 8.75, **p<0.001 versus saline-treated mice). (C) Effect of spadin (i.p., 10−5 M) and diazepam (i.p., 0.5 mg/kg) on the total number of entries in the aversive white side in the light/dark transition test (n = 10 per group, one-way ANOVA, F2,53 = 7.65, ***p = 0.001 versus saline-treated mice). (D) Influence of spadin (i.p., 10−5 M) and diazepam (i.p., 0.5 mg/kg) on mouse performance in the staircase test. Data are presented as the ratio of number of rearings over the number of ascended steps (n = 10 per group, one-way ANOVA, F2,44 = 4.86, *p<0.05 versus saline-treated mice). In the three tests, mice were injected with either spadin or diazepam 30 min before the test. In all graphs, bars indicate SEM.