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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Exp Hematol. 2010 Feb 17;38(4):311–320. doi: 10.1016/j.exphem.2010.02.005

Figure 1. Generation of human pancreas derived MSC by in vitro three-step culture system.

Figure 1

(A) Culture of human fetal pancreatic cells in restrictive medium (RPMI 1640 with 0.5% FBS) eliminated the contaminating cells and allowed survival of the pMSC which acquired characteristic spindle-shaped morphology (original magnification × 40).

(B) Expansion medium (RPMI 1640 with 10% FBS and growth factors) induced rapid proliferation of the pancreas-derived mesenchymal stem cells (pMSC). Cells are shown at passage 7 (original magnification, × 10). (C) pMSC growth rate in culture. The x-axis represents the number of incubation days and the y-axis represents cumulative cell numbers.