Table 2. Human C-peptide concentrations in sheep serum following fetal transplantation with human fetal pancreatic mesenchymal stem cells.
Five of ten transplanted sheep had detectable levels of human C-peptide at varying intervals post-transplantation. All samples were obtained after fasting for 24 hr.
| Animal no. |
When sampled (months post-transplant) |
Human C-peptide (ng/mL) |
CV% |
|---|---|---|---|
| 2349 | 7 | 0.43 | ± 5% |
| 25 | 2.73 | ± 10% | |
| 27 | 0.75 | ± 8% | |
| 2351 | 7 | ND | |
| 25 | ND | ||
| 27 | ND | ||
| 2352 | 7 | 0.47 | ± 1% |
| 25 | 0.36 | ± 9% | |
| 27 | 0.32 | ± 1% | |
| 2353 | 7 | ND | |
| 25 | ND | ||
| 2354 | 7 | ND | |
| 2356 | 7 | 1.86 | ± 14% |
| 25 | 1.46 | ± 6% | |
| 27 | 0.83 | ± 4% | |
| 2358 | 7 | ND | |
| 25 | ND | ||
| 2359 | 7 | ND | |
| 25 | ND | ||
| 27 | ND | ||
| 2360 | 7 | 1.21 | ± 1% |
| 25 | 4.49 | ± 2% | |
| 27 | ND | ||
| 2362 | 7 | 0.76 | ± 6% |
| 25 | 0.56 | ± 5% | |
| 27 | ND |
Human C-peptide assay (ELISA) standards ranged from 0.5-20 ng/mL with a detection limit of 0.30 ng/mL. Error is reported as the coefficient of variation (%). All control (non-transplanted, n=5) sheep failed to express human C-peptide. ND: not detected.