Table 1.

Kinetic Analysis of Wild-Type and 3′-dATP Reactions^a

	ATP	3′-dATP
kcat (s−1)	3.2 ± 0.5	0.045 ± 0.02 (71)b
kobs (s−1)	28 ± 2	0.6 ± 0.08 (47)
Km (ATP/3′-dATP) (mM)	0.2 ± 0.02	0.41 ± 0.03 (2)
Km (GLN) (mM)	0.26 ± 0.04	4.24 ± 0.01 (16)
kcat/Km (GLN) (s−1 M−1)	1.2 × 104	11.3 (1060)

^aValues for wild-type GlnRS are taken from ref 26.

^bNumbers in parentheses designate the fold diminution from wild-type for k_cat, k_cat/K_m, and k_obs, and the fold elevation from wild-type for K_m.