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. 2010 Apr 13;5(4):e10163. doi: 10.1371/journal.pone.0010163

Figure 2. Tetracycline-dependent repression of the IRP2 transgene abolishes accelerated tumor growth.

Figure 2

A total of 6 BALB/c nude mice were injected with HIRP2wt cells to form tumor xenografts. Half of the animals were receiving 2 mg/ml tetracycline in the drinking water throughout the experimental period, starting 4 days before injection. (A) Representative anesthetized mice from the two groups before sacrifice (tumors shown by arrows). (B) Kinetics on tumor xenograft growth. (C) Mass and (D) volume of isolated tumor xenografts. (E) Tumor tissue extracts were analyzed by Western blotting with antibodies against HA, to detect expression of the IRP2 transgene, and β-actin, as loading control. Data are expressed as mean ± SEM. * p<0.05, ** p<0.01 versus HIRP2wt (Student's t-test).