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. 2010 Apr 13;5(4):e10163. doi: 10.1371/journal.pone.0010163

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Maffettone et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Extracts from tumor tissue were analyzed by Western blotting with antibodies against HA, TfR1, ferritin, ferroportin, DMT1 and β-actin. (B) Tumor extracts were analyzed for IRE-binding activity by EMSA with a ³²P-labeled ferritin IRE probe. (C and D) Analysis of TfR1 and H-ferritin mRNA expression by qPCR.