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. 2010 Apr 13;5(4):e10166. doi: 10.1371/journal.pone.0010166

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) DAPI staining showing all nuclei of the 3^rd instar wing disc, Gro staining showing dorsal wing pouch overexpression (driven by MS1096-Gal4 in females), and β-gal staining showing expression of the vestigial quadrant enhancer that is bisected by the dorsoventral boundary. Top row: β-gal staining showing vgQ-lacZ transgene expression in a 3^rd instar wing disc under wildtype conditions. Middle row: β-gal staining showing a reduction of vgQ-lacZ expression in the dorsal half of the wing pouch where it colocalizes with Gro overexpression Bottom row: β-gal staining showing an expanded vgQ-lacZ expression in the presence of Gro overexpression when raised on food containing 20 µM TSA. <D/V> is the average value (± standard deviation) for the length of the dorsal stripe divided by the length of the ventral stripe of vgQ-lacZ expression. (B) <D/V> as a function of TSA concentration. Increasing TSA concentration results in a dose dependent decrease in Gro-mediated repression of the vgQ-lacZ transgene. The p-value for the slope of the line is less than 0.01. The following numbers of wings were examined at the given TSA concentrations: DMSO alone (n = 71), 10 µM (n = 46), 12 µM (n = 59), 14 µM (n = 48), 16 µM (n = 35), 18 µM (n = 31), 20 µM (n = 15). (C) <D/V> ratio as a function of HC-Toxin concentration. Increasing HC-Toxin concentration also results in a dose dependent decrease in Gro-mediated repression on the vgQ-lacZ transgene. The p-value for the slope of the line is less than 0.01. The following number of wings were examined at the given HC-Toxin concentrations: DMSO alone (n = 71), 10 µM (n = 60), 12 µM (n = 27), 14 µM (n = 18), 16 µM (n = 14), 18 µM (n = 5), 20 µM (n = 6).