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. 2010 Jan 28;49(8):1596–1605. doi: 10.1021/bi9019912

Figure 2.

Figure 2

Ensemble and single-molecule FRET analyses of the P1−P2 aptamer domain variant. (A) Design of P1−P2 for smFRET analysis. For ensemble FRET, the neutravidin surface was omitted. (B) Ensemble FRET titration of P1−P2 with Mg2+ at 0 or 500 nM guanine. (C) smFRET histograms for P1−P2. The vertical guidelines are at E values of 0.32 and 0.47. (D) Representative single-molecule smFRET time traces for P1−P2.