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. Author manuscript; available in PMC: 2011 Apr 9.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Mar 11;394(3):667–672. doi: 10.1016/j.bbrc.2010.03.047

Fig. 3. Knockdown of EGFP expression using lentivirus in HeLa cells.

Fig. 3

A. HeLa cells transduced with 5 MOI of pcFUW-LUCkd-pgk-puro control vector and 1 MOI of pcFUW-EGFP cotransduced HeLa cells in light field. B. EGFP green fluoresence visualized from A. C: Morphology of HeLa cells transduced with 5 MOI of pcFUW-EGFPkd-pgk-puro and 1 MOI of control vector pcFUW-EGFP in light field. D. EGFP expression from C was silenced under fluorescence microscopy. E. EGFP expression in HeLa cells was detected by Western blot after co-transduction with 1 MOI pcFUW-EGFP with 15 MOI control vector pcFUW-miR21LUCkd-pgk-puro (I) and different MOI of EGFP knockdown viruses pcFUW-miR21EGFPkd 5(II), 10(III) and 15(IV), respectively.