Table 2. FGFs induce Wnt8a expression in chick explant culture.
Explants of chick HH stage 4-5 rostral ectoderm were cultured in the absence (control) or presence of FGF protein-soaked beads and assayed for chick Wnt8a expression. The fraction of all explants showing any Wnt8a expression is indicated in the first column, and the number of explants exhibiting strong (++), weak (+), or no (-) expression of Wnt8a is indicated is indicated in subsequent columns.
| Beads | Wnt8a pos./total | Wnt8a ++ | Wnt8a + | Wnt8a - | 
|---|---|---|---|---|
| Control | 1/10 | 0 | 1 | 9 | 
| FGF4 | 11/12 | 10 | 1 | 0 | 
| FGF3 | 4/12 | 0 | 4 | 8 | 
| FGF10 | 1/12 | 0 | 1 | 11 | 
++ = strong expression
+ = weak expression
- = no expression