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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: J Neurosci Methods. 2010 Feb 12;188(2):195–204. doi: 10.1016/j.jneumeth.2010.02.007

Figure 7. Requirement of rutabaga and the MB in the formation of memory induced by the apparatus.

Figure 7

(A) One hour-memory was measured in Canton-S (CS) or rutabaga mutant flies that were trained with pulsed or constant flow protocol. Apparent reduction in memory was observed in rutabaga mutant (n=8 for each, ***p < 0.001, t-test). Pulsed flow gave higher memory score than constant flow in CS or rutabaga mutants (*p<0.05). (B) Flies impaired in MB-output were trained with pulsed or constant flow protocol and 1-hr memory was measured. Neurotransmissions from the MBs were inhibited by a temperature-sensitive form of shibire transgene (shits), which was driven by MB-specific driver Gal4–247. Flies possessing both shits and Gal4–247 showed lower 1 hr-memory at 32 °C than flies having only shits or Gal4–247 (n=6, ***p<0.001, ANOVA). Pulsed flow gave higher memory score than constant flow in control flies or flies impaired in MB-output (n=6, *p<0.05, **p<0.01, t-test). (C) Flies subjected to Hydroxyurea (HU) were trained with pulsed flow protocol. MB-ablated flies with HU resulted in lower 1 hr memory than control flies (n=6, ***p<0.001, t-test).