(A) Flies were conditioned by the spaced training protocol with pulsed flow (IPI= 5 s or 10 s) or constant flow. The pulsed flow protocol induced an identical level of LTM to that with constant flow. (B) Repeated training induced strong 24 hr memory. Memory retention became greater in proportion to the number of training cycles (n=6, *p < 0.05, ANOVA). (C) Spaced training with 15 min interval between training sessions induced stronger 24 hr memory than massed training in which training sessions were repeated without interval. (n=6, *p <0.05, ***p <0.001, ANOVA). Data are presented as mean ±SEM.