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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Eye Res. 2010 Feb 4;90(5):555–563. doi: 10.1016/j.exer.2010.01.012

Fig 2. Inhibition of AR prevents LPS-induced expression of inflammatory marker proteins.

Fig 2

Growth-arrested hNPECs were preincubated with sorbinil or zopolrestat for 24 h followed by treatment with LPS (1 μg/ml) and incubation for additional 24 h. At the end of incubation, cells were lyzed and equal amount of protein (40 μg) was separated on SDS-PAGE followed by immunoblotting using antibodies against COX-2, iNOS, and ICAM-1. Immunoblotting of the stripped membranes with GAPDH antibodies was used to depict equal protein loading. Numbers below the blots show fold changes in the expression of proteins (n=3). sorb, sorbinil; zopol, zopolrestat.