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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Eye Res. 2010 Feb 4;90(5):555–563. doi: 10.1016/j.exer.2010.01.012

Fig 4. Inhibition of AR attenuates LPS-induced phosphorylation of (A) MAP-Kinases and (B) PLC-β3 and PKC-βII in hNPECs.

Fig 4

(A) Growth-arrested hNPECs were preincubated with sorbinil, zopolrestat or carrier for 24 h followed by treatment with LPS (1 μg/ml) and incubation for different time periods as indicated. At the end of incubation, cells were lysed and equal amount of protein (40 μg) was separated on SDS-PAGE followed by immunoblotting using antibodies against phospho- and total p38, ERK1/2, and SAPK/JNK. Immunoblotting of the stripped membranes with GAPDH antibodies was used to depict equal protein loading. (B) Growth-arrested hNPECs were preincubated with zopolrestat for 24 h followed by treatment with LPS (1 μg/ml) for different durations as indicated. At the end of incubation, cells were harvested, cell membrane fraction was prepared and equal amount of protein (15 μg) was separated on SDS-PAGE followed by immunoblotting using antibodies against phospho- PLCβ3 and -PKC-βII. Immunoblotting of the stripped membranes with antibodies against pan-cadherin was performed to show equal loading of protein (n=3). zopol, zopolrestat.