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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Eye Res. 2010 Feb 4;90(5):555–563. doi: 10.1016/j.exer.2010.01.012

Fig 6. Inhibition of AR prevents HNE- and GS-HNE-induced activation of NF-κB but not by GS-DHN in hNPECs.

Fig 6

Growth-arrested hNPECs were incubated with AR inhibitor for over-night and transfected with NF-κB-pSEAP vector or control (pTAL SEAP) vector using Lipofectamine 2000. After 6 h, medium was replaced with fresh medium and cells were stimulated with HNE, GS-HNE-ester or GS-DHN-ester (1 μM each) and incubated for 48 h with or without AR inhibitor. Cell culture media were harvested, cleared by centrifugation and NF-κB-dependent reporter SEAP activity was measured by chemiluminescence’s method essentially as described by the manufacturer. *p<0.001 Vs Control; **p<0.01 Vs HNE or GS-HNE-ester alone (n=3).