Table I.
Enzyme yield in transplastomic tobacco plants
| Enzyme | Leaf age |
No of leaves /plant |
Avg. Wt (g)/leaf |
Units/ g in fresh leaf |
Units | Whole plant yield |
Units(millions)/ acre/ cutting |
Units(millions) /acre/year |
|
|---|---|---|---|---|---|---|---|---|---|
| Per leaf | Per age group |
||||||||
| PelB | Young | 3.5 | 2.5 | 20.82 | 52.05 | 182.18 | 8.89 % | 16.39 | 49.17 |
| Mature | 8.2 | 8.0 | 25.56 | 204.48 | 1,676.74 | 81.84% | |||
| Old | 4.5 | 5.6 | 7.54 | 42.22 | 190.00 | 9.27% | |||
| PelD | Young | 3.5 | 2.5 | 26.65 | 66.63 | 233.19 | 8.70% | 21.43 | 64.3 |
| Mature | 8.2 | 8.0 | 32.44 | 259.52 | 2,128.06 | 79.43% | |||
| Old | 4.5 | 5.6 | 12.62 | 70.67 | 318.02 | 11.87% | |||
| CelD | Young | 3.5 | 2.5 | 3,000 | 7,500 | 26,250 | 5.86% | 3,583.50 | 10751 |
| Mature | 8.2 | 8.0 | 4,930 | 39,440 | 323,408 | 72.20% | |||
| Old | 4.5 | 5.6 | 3,900 | 21,840 | 98,280 | 21.94% | |||
One unit of PelB and PelD enzyme is defined as the amount of enzyme which forms 1 μmol of unsaturated soluble oligogalacturonates per min with a molar extinction coefficient of 4,600 μmol−1 cm−1 in a 2.5 ml reaction containing 2.5 mg/ml polygalacturonic acid.
One unit of CelD enzyme is defined as the amount of enzyme that released 1 μmole glucose equivalents per minute in a 1ml reaction containing 2%CMC.