Fig. 2.

Developmental profile of tenectin expression. Northern blot hybridization of RNA isolated from staged late third instar larvae, prepupae, pupae and unstaged adults. The probe used for hybridization was prepared from the common fifth exon. The blot was reprobed to detect an early gene (E74) and a prepupal gene (β-Ftz-F1). Previously identified peaks in ecdysone titer are shown (Richards, 1981; Handler, 1982; Warren et al., 2006). Developmental times are shown on top, in hours after puparium formation (APF). Hybridization to detect rp49 mRNA (O’Connell and Rosbash, 1984) was used as a control for loading and transfer. This experiment was performed twice with very similar results (data not shown).