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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Dev Biol. 2010 Feb 10;340(2):504–517. doi: 10.1016/j.ydbio.2010.02.008

Fig. 3.

Fig. 3

Stimulation of tenectin transcription by 20E. (A) tenectin RNA levels are shown for mass isolated third instar larval imaginal discs maintained in culture without added hormone (black bars) or treated with 5 × 10−6 M 20E (grey bars) for 0, 3 or 6 hours. (B) tenectin mRNA levels in larval organs cultured for 6 hours in the absence (C) or presence of 20E alone, 20E and cycloheximide (20E+Cy), or cycloheximide (Cy) alone. Total RNA was analyzed by Northern blot hybridization and tenectin mRNA was quantified by volume integration densitometry. Hybridization to detect rp49 mRNA (O’Connell and Rosbash, 1984) was used as a control for loading and transfer. Error bars are s.e.m. and asterisks indicate significant differences from control using Student’s t test (P<0.01).