Figure 1. Diagram and summary of activity of Neurog1-GFP transgenes.

Comparison of mouse and human genomes surrounding the Neurog1 coding sequence on mouse chromosome 13 reveals extensive conservation (shown 50–100%) in non-coding regions using ECR browser (Ovcharenko et al., 2004). Colors indicate over 70% conservation in sequence where blue is Neurog1 coding, yellow is UTR, and red is intergenic. Black blocks below the ECR diagram indicate sequence conserved to D. rerio that have been identified in functioning enhancers (LATE, ANPE, LSE) (Blader et al., 2004; Blader et al., 2003). The BAC transgene N1⁴⁵⁷-nGFP (modified BAC RP23 457E22) is shown with the location of deletions in ΔR1, ΔR2, ΔR3 indicated by brackets. The relative location of the deletions and the sequences tested in the transgenes are diagramed below the ECR browser image to highlight the conserved regions included in each. Precise coordinates in the genome are given in Table 1. Green boxes indicate the GFP reporter coding sequence. # Expressing indicates the number of independent transgenic founder embryos at E11.5 that had detectable GFP and were examined for expression in the tissues listed. Expression pattern was consistent across embryos with the same transgene and representative images are shown in Figs. 2 and 4. Black +/− indicate similarity to wildtype while red +/− highlight expression different from wildtype. The asterisks on TgN1-2 and TgN1-13dnt indicate they were reported in Nakada et al. 2004 and are shown here for comparison. ^indicates these transgenes aberrantly directed ventral telencephalon (vt) expression rather than dorsal. vnt, ventral neural tube; dnt, dorsal neural tube.