Fig. 4.
pOBCol3.6GFPtpz and pOBCol2.3GFPemd expression in calvarial cell cultures. Calvarial osteoblasts with GFP driven by a 3.6-kb Col1a1 promoter (pOBCol3.6GFPtpz) from Sh3bp2+/+, Sh3bp2+/KI, and Sh3bp2KI/KI mice showed no difference in GFP activity on day 7 of osteoblast differentiation (A); Calvarial osteoblasts with GFP driven by a 2.3-kb Col1a1 promoter (pOBCol2.3GFPemd) from Sh3bp2KI/KI mice showed reduced GFP activity compared to Sh3bp2+/+ samples on day 18 of osteoblast differentiation (B). BF: bright field; 3.6GFP: GFPtpz filter; 2.3GFP: GFPemd filter.
