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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Hematol. 2010 Mar 3;38(5):403–416. doi: 10.1016/j.exphem.2010.02.011

Figure 4.

Figure 4

(A) Over-expression of RUNX1 isoforms in mouse hematopoietic stem/progenitor cells does not affect homing to the bone marrow. Analysis of the bone marrow of recipient mice 24-hours post-transplant of transduced hematopoietic progenitors showed that there was no difference in the total number of donor cells (CD45.2+) or transduced donor cells (CD45.2+GFP+) in the bone marrow of recipient mice. (B) The spleen CFU-S colony assay demonstrated that although there was no difference in the number of GFP− spleen colonies in mice transplanted with transduced cells, no GFP+ colonies were detected from cells over-expressing RUNX1 isoforms. (C) Over-expression of RUNX1 isoforms in fetal liver HSCs. Transplantation of transduced fetal liver HSCs showed that while there was no difference in total number of donor-derived peripheral blood cells (CD45.2+), essentially no peripheral blood progeny (CD45.2+GFP+) could be detected from HSCs over-expressing RUNX1 isoforms.