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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 Nov 13;48(5):989–998. doi: 10.1016/j.yjmcc.2009.11.004

Fig. 8.

Fig. 8

The effect of the D166V mutation on RLC phosphorylation in transgenic mouse ventricular myofibrils blotted with +P-human RLC antibody recognizing phosphorylated human RLC (transgenic). Lane 1, +P WT st, phosphorylated recombinant standard WT protein; lane 2, Tg-WT RV, transgenic wild type myofibrils isolated from right ventricles of Tg-WT mice; lane 3, Tg-D166V RV, transgenic D166V mutant myofibrils isolated from right ventricles of Tg-D166V mice; D166V st, non-phosphorylated recombinant human D166V standard protein. Myosin essential light chain (ELC) served as a loading control and was detected with monoclonal MAB150 antibody.